Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

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We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution.It involves melting a cryo sample with a laser beam to allow dynamics of the click here embedded particles to occur.Once the laser beam is switched off, the sample revitrifies within just a few microseconds, trapping the particles in their transient configurations, which can subsequently be imaged to obtain a snap shot of the dynamics at this point in time.While we have previously turbo air m3f24-1 performed such experiments with a modified transmission electron microscope, we here demonstrate a simpler implementation that uses an optical microscope.

We believe that this will make our technique more easily accessible and hope that it will encourage other groups to apply microsecond time-resolved cryo-EM to study the fast dynamics of a variety of proteins.